Publications
Technology Overview
Expert Opin Ther Targets 2006;10(1):179-190.
Transcreener™: screening enzymes involved in covalent regulation
Robert G Lowery & Karen Kleman-Leyer
Transcreener™: screening enzymes involved in covalent regulation
Robert G Lowery & Karen Kleman-Leyer
ADP Assay
Mol Cell. 2011 Mar 4;41(5):567-78.
Structure of lipid kinase p110ß/p85ß elucidates an unusual SH2-domain-mediated inhibitory mechanism.
Zhang X, Vadas O, Perisic O, Anderson KE, Clark J, Hawkins PT, Stephens LR, Williams RL.
Structure of lipid kinase p110ß/p85ß elucidates an unusual SH2-domain-mediated inhibitory mechanism.
Zhang X, Vadas O, Perisic O, Anderson KE, Clark J, Hawkins PT, Stephens LR, Williams RL.
Molecular and Biochemical Parasitology 2011 Jan: 175(1):21-29
Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds
Crowther GJ, Napuli AJ, Gilligan JH, Gagaring K, Borboa R, Francek C, Chen Z, Dagostino EF, Stockmyer JB, Wang Y, Rodenbough PP, Castaneda LJ, Leibly DJ, Bhandari J, Gelb MH, Brinker A, Engels IH, Taylor J, Chatterjee AK, Fantauzzi P, Glynne RJ, Van Voorhis WC, Kuhen KL.
Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds
Crowther GJ, Napuli AJ, Gilligan JH, Gagaring K, Borboa R, Francek C, Chen Z, Dagostino EF, Stockmyer JB, Wang Y, Rodenbough PP, Castaneda LJ, Leibly DJ, Bhandari J, Gelb MH, Brinker A, Engels IH, Taylor J, Chatterjee AK, Fantauzzi P, Glynne RJ, Van Voorhis WC, Kuhen KL.
J Biomol Screen. 2010 Mar;15(3):279-86. Epub 2010 Feb 10.
Detection of the ATPase activity of the molecular chaperones Hsp90 and Hsp72 using the TranscreenerTM ADP assay kit.
Rowlands M, McAndrew C, Prodromou C, Pearl L, Kalusa A, Jones K, Workman P, Aherne W.
Detection of the ATPase activity of the molecular chaperones Hsp90 and Hsp72 using the TranscreenerTM ADP assay kit.
Rowlands M, McAndrew C, Prodromou C, Pearl L, Kalusa A, Jones K, Workman P, Aherne W.
J Biomol Screen 2009; 14(7): 838-844
High-Throughput, Cell-Free, Liposome-Based Approach for Assessing In Vitro Activity of Lipid Kinases
Douglas J. Demian, Susan L. Clugston, Meta M. Foster, Lucia Rameh, Deborah Sarkes, Sharon A. Townson, Lily Yang, Melvin Zhang, Maura E. Charlton
High-Throughput, Cell-Free, Liposome-Based Approach for Assessing In Vitro Activity of Lipid Kinases
Douglas J. Demian, Susan L. Clugston, Meta M. Foster, Lucia Rameh, Deborah Sarkes, Sharon A. Townson, Lily Yang, Melvin Zhang, Maura E. Charlton
J Biomol Screen 2009; 14(6): 679-689
Efficient Elimination of Nonstoichiometric Enzyme Inhibitors from HTS Hit Lists
Michael Habig, Anke Blechschmidt, Sigmar Dressler, Barbara Hess, Viral Patel, Andreas Billich, Christian Ostermeier, David Beer, Martin Klumpp
Efficient Elimination of Nonstoichiometric Enzyme Inhibitors from HTS Hit Lists
Michael Habig, Anke Blechschmidt, Sigmar Dressler, Barbara Hess, Viral Patel, Andreas Billich, Christian Ostermeier, David Beer, Martin Klumpp
Comb Chem High Throughput Screen 2009; 12(3):258-68
Development and Validation of a High-density Fluorescence Polarization-based Assay for the Trypanosoma RNA Triphosphatase TbCet1
Antczak C, Shum D, Radu C, Seshan VE, Djaballah H.
Development and Validation of a High-density Fluorescence Polarization-based Assay for the Trypanosoma RNA Triphosphatase TbCet1
Antczak C, Shum D, Radu C, Seshan VE, Djaballah H.
Assay Drug Dev Technol 2009; 7(1):56-67
Characterization and Optimization of a Red-Shifted Fluorescence Polarization ADP Detection Assay
Karen M. Kleman-Leyer, Tony A. Klink, Andrew L. Kopp, Thane A. Westermeyer, Mark D. Koeff, Brad R. Larson, Tracy J. Worzella, Cori A. Pinchard, Sebastianus A.T. van de Kar, Guido J.R. Zaman, Jorrit J. Hornberg, Robert G. Lowery
Characterization and Optimization of a Red-Shifted Fluorescence Polarization ADP Detection Assay
Karen M. Kleman-Leyer, Tony A. Klink, Andrew L. Kopp, Thane A. Westermeyer, Mark D. Koeff, Brad R. Larson, Tracy J. Worzella, Cori A. Pinchard, Sebastianus A.T. van de Kar, Guido J.R. Zaman, Jorrit J. Hornberg, Robert G. Lowery
J Biomol Screen 2008; 13(6):476-485
Evaluating PI3 Kinase Isoforms Using Transcreener™ ADP Assays
Klink TA, Kleman-Leyer K, Kopp A, Westermeyer TA, Lowery RG
Evaluating PI3 Kinase Isoforms Using Transcreener™ ADP Assays
Klink TA, Kleman-Leyer K, Kopp A, Westermeyer TA, Lowery RG
J Biomol Screen 2007; 12(4):578-584
Development of a Transcreener™ Kinase Assay for Protein Kinase A and Demonstration of concordance of Data with a Filter-Binding Assay Format
Karen L. Huss, Pauline E. Blonigen, and Robert M. Campbell
Development of a Transcreener™ Kinase Assay for Protein Kinase A and Demonstration of concordance of Data with a Filter-Binding Assay Format
Karen L. Huss, Pauline E. Blonigen, and Robert M. Campbell
Assay Drug Dev Technol 2007; 5:225-235
Discovery of Acetyl-Coenzyme A Carboxylase 2 Inhibitors: Comparison of a Fluorescence Intensity-Based Phosphate Assay and a Fluorescence Polarization-Based ADP Assay for High-Throughput Screening
Yichin Liu, Leeanne Zalameda, Ki Won Kim, Minghan Wang, John D. McCarter
Discovery of Acetyl-Coenzyme A Carboxylase 2 Inhibitors: Comparison of a Fluorescence Intensity-Based Phosphate Assay and a Fluorescence Polarization-Based ADP Assay for High-Throughput Screening
Yichin Liu, Leeanne Zalameda, Ki Won Kim, Minghan Wang, John D. McCarter
AMP/GMP Assay
J Biomol Screen. January 2012 vol. 17 no. 1 59-70
Development and Validation of a Generic Fluorescent Methyltransferase Activity Assay Based on the Transcreener AMP/GMP Assay.
Klink TA, Staeben M, Twesten K, Kopp AL, Kumar M, Schall Dunn R, Pinchard CA, Kleman-Leyer KM, Klumpp M, Lowery RG.
Development and Validation of a Generic Fluorescent Methyltransferase Activity Assay Based on the Transcreener AMP/GMP Assay.
Klink TA, Staeben M, Twesten K, Kopp AL, Kumar M, Schall Dunn R, Pinchard CA, Kleman-Leyer KM, Klumpp M, Lowery RG.
Science. 2011 Jul 22;333(6041):453-6
De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila.
Neunuebel MR, Chen Y, Gaspar AH, Backlund PS Jr, Yergey A, Machner MP.
De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila.
Neunuebel MR, Chen Y, Gaspar AH, Backlund PS Jr, Yergey A, Machner MP.
Assay Drug Dev Technol. 2010 Jun;8(3):344-55
Development and Validation of a Transcreener Assay for Detection of AMP- and GMP-Producing Enzymes
Staeben M, Kleman-Leyer KM, Kopp AL, Westermeyer TA, Lowery RG.
Development and Validation of a Transcreener Assay for Detection of AMP- and GMP-Producing Enzymes
Staeben M, Kleman-Leyer KM, Kopp AL, Westermeyer TA, Lowery RG.
GDP Assay
J Biomol Screen 2009;
Two Gαi1 Rate-Modifying Mutations Act in Concert to Allow Receptor-Independent, Steady-State Measurements of RGS Protein Activity
Thomas Zielinski, Adam J. Kimple, Stephanie Q. Hutsell, Mark D. Koeff, David P. Siderovski, Dr. , and Robert G. Lowery
Two Gαi1 Rate-Modifying Mutations Act in Concert to Allow Receptor-Independent, Steady-State Measurements of RGS Protein Activity
Thomas Zielinski, Adam J. Kimple, Stephanie Q. Hutsell, Mark D. Koeff, David P. Siderovski, Dr. , and Robert G. Lowery
J Biomol Screen. 2011 Aug;16(7):717-23. Epub 2011 May 18
High-throughput fluorescence polarization assay for the enzymatic activity of GTPase-activating protein of ADP-ribosylation factor (ARFGAP).
Sun W, Vanhooke JL, Sondek J, Zhang Q.
High-throughput fluorescence polarization assay for the enzymatic activity of GTPase-activating protein of ADP-ribosylation factor (ARFGAP).
Sun W, Vanhooke JL, Sondek J, Zhang Q.
Curr Protoc Pharmacol. 2012 Mar;Chapter 2:Unit2.8.
Bosch DE, Zielinski T, Lowery RG, Siderovski DP.
UDP Assay
Chembiochem. 2010 Sep 24;11(14):1939-49. Review
Glycosyltransferases and their assays.
Wagner GK, Pesnot T.
Glycosyltransferases and their assays.
Wagner GK, Pesnot T.