• Complete Biochemical Assay Solutions for Your Toughest Enzyme Targets

    Whether you are screening a million compounds, prioritizing hits from a virtual screen, or optimizing a lead molecule, our enzyme assay solutions generate the high-quality data you need to advance your program.

    Run proven assays in your lab or work with BellBrook scientists to establish, troubleshoot, and validate a biochemical workflow before screening, hit confirmation, or CRO transfer.

    Right-open Right-open Find the Right Assay Right-open Right-open Validate an Assay Workflow

How Can BellBrook Help?

Check Mark Icon

I need an assay or enzyme.

Find assay kits, active enzymes, and complete assay systems for running biochemical assays in your lab.

Right-open Right-open Find the Right Assay
Protocol Icon

I need help validating a workflow.

Evaluate assay feasibility, troubleshoot performance, establish controls, and determine whether a workflow is ready for screening or compound validation.

Right-open Right-open Explore the Assay Validation Sprint
Publications Icon

I want BellBrook to run the work.

Access assay development, compound testing, dose-response studies, hit validation, and profiling without adding internal resources.

Right-open Right-open Explore Scientific Services

Robust, Reliable Biochemical Assays and Comprehensive, Scientific Support

Active Enzymes Icon

Active Enzymes

Highly sensitive, active human enzymes validated for HTS in our proprietary assays: cGAS, TREX1, PolQ, WRN, PARG, CD38, DDX helicases, MDA5, RIG-I, and many more.

Assays Icon

Robust HTS Assays

Mix-and-Read assays for Kinases, ATPases, GTPases, methyltransferases, phosphodiesterases, glycosyltransferases, DDR enzymes and immune sensors with first-in-class sensitivity and performance.

Reagents Icon

Reagents and Protocols

Validated substrates, cofactors, and buffers with detailed protocols for inhibitor screening, dose-response, and mechanistic studies.

Support Icon

Outstanding Support

Instant quotes, next-day delivery, and collaborative technical support from our experienced assay development / enzymology scientists.

Assay System
Right Arrow
Mix-and-Read Assay Outline
Right Arrow
Compound Screening Data
Right Arrow
Dose Response Data

Our Technology

We combine innovative HTS assay platforms with domain expertise in protein biochemistry and enzymology to deliver enzyme, assay solutions that you can rely on.

All our assays are designed to produce robust results in an automated HTS environment on common multimode readers.

Check Mark Icon

Enzolution

The complete assay solution for a wide range of scientific discoveries.


Learn More
  • Validated enzymes, substrates, buffers, and protocols, ready for inhibitor discovery
  • Designed to be used with the Transcreener and AptaFluor platforms
  • Enzolution is the one-stop-shop for your biochemical assay needs

Transcreener

The simplest, most robust HTS platform available for nucleotide-dependent enzymatic activity.

Learn More
  • The only HTS method for direct detection of nucleotides produced by enzymes
  • Detects thousands of enzymes: kinases, ATPases, GTPases, phosphodiesterases, glycosyltransferases, exonucleases, etc.
  • Continuous OR end point assays
  • True single-step mix-and-read format

AptaFluor

We offer the simplest and most sensitive methyltransferase available.

Learn More
  • Mix-and-read SAH detection technologies
  • Detect any methyltransferase using SAM at Km concentration
  • Use with virtually any methyltransferase enzyme

Enzymes

Purified, active enzymes validated in our assays

Learn More
  • Purified enzymes with high enzyme activity assays
  • Validated and optimized in BellBrook’s assays
  • Priced for scaling to HTS

Services

Let our experienced scientists handle even your most demanding targets

Discover Our Services
  • Industry leading turnaround times for your convenience
  • Custom assay conditions to fit every study
  • Proven Transcreener technology

Why Rely on Us for Your Biochemical Assay Needs?

Customer Support Icon

Highest Quality Customer Support

Our customer experience is a cut above the rest—we pride ourselves on being fast to respond and easy to work with. With BellBrook Labs, you can look forward to:

  • Instant quotes and next day delivery for most products
  • Next day delivery for domestic orders
  • We’re always ready to provide fast, friendly, and comprehensive responses to any questions you have.
Shopping Cart Icon

Complete Solutions, Minus the Complexity

BellBrook Labs offers everything you need in one place. We deliver robust, high-sensitivity signals, eliminating the need for cumbersome detection chemistry

  • Validated enzymes, cofactors, and substrates
  • Detailed protocols for inhibitor screening and dose response
  • Papers, Posters, Application Notes and E-books to support your needs
Technical Support Icon

Customer-Centric, Scientific Expertise

BellBrook Labs is a scientist-led company. We’ve been developing biologically relevant, innovative enzyme assays for more than 20 years. Here’s how we do it:

  • Our support team is led by the same scientists who develop the assays we offer.
  • We provide same day thorough and informed responses to all technical inquiries
  • We’ll collaborate directly with your team to help ensure the success of your study

Proven Results

Since 2005, our technologies have been trusted all over the world by pharmaceutical companies, tech companies, and academic labs.

Biochemical assays provided by BellBrook Labs have helped make new scientific discoveries possible in the following fields:

  • Cancer
  • Autoimmune disease
  • Neurodegeneration
  • Infectious diseases

“Some CROs reserve their best teams for high profile customers. If you’re not right there with them you get less experienced teams… BellBrook understands enzyme kinetics, so it made that part of the project so much easier. The larger CRO didn’t seem to have the expertise we needed in this area.”

“The ADP assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.”

“Excellent blend of simplicity & good science. Excellent technical support”

“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook knows what they are doing.”

“Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.”

“The Transcreener AMP/GMP Assay is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay I needed 1 µM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with the assay development. Their representative has been very willing to quickly help me get my assay up and running.”

“We were struggling with a narrow assay window and poor sensitivity with luciferase-coupled ATPase assays. Initially, we had Bellbrook run their Transcreener ATPase assay in-house as a contracted project. We were so pleased with the results that we immediately onboarded the assay to our company lab. Throughout the whole process, Bellbrook scientists were clear, rapid communicators and demonstrated their deep knowledge of the assay. We highly recommend Bellbrook to our peers in drug discovery.”

PreviousNext

Resources

Ready to learn more and advance your study? Find the right resources to help you get to your solution faster—including posters, details on Instrument capability, FAQs and more.

Link to: Frequently Asked Questions (FAQ’s)
FAQ Icon

FAQ

Link to: Technical Manuals
Protocol Icon

Protocols

Link to: Publications
Publications Icon

Publications

Link to: Transcreener® Assays – Instrument Compatibility
Instrument Compatibility Icon

Instrument Compatibility

Link to: Application Notes
Application Notes Icon

Application Notes

Link to: Posters and Presentations
Posters Icon

Posters

Contact Us

This field is for validation purposes and should be left unchanged.
Name(Required)

Latest Articles

Explore the latest articles on HTS assays for cancer drug discovery, hit to lead assays, new tools for ATPase Drug Discovery and more.

Managing high-throughput workflow in the lab

Why HTS Campaigns Slow Down After Assay Development (and How to Keep Them Moving)

Quick Answer: Even well-developed assays can lead to delays in high-throughput screening (HTS) campaigns due to: Scaling challenges from development to screening formats Data quality issues under real screening conditions False positives that slow hit validation Workflow complexity limiting…
Read more
Microplate reagent analysis and workflow infographic

How CROs Can Reduce Screening Costs Without Sacrificing Data Quality

Quick Answer: Contract research organizations (CROs) can reduce high-throughput screening (HTS) costs without sacrificing data quality by focusing on four key levers: Improve reagent efficiency to reduce cost per well Select assay formats that minimize interference and rework Reduce false…
Read more
BellBrook Labs logo

BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400

info@bellbrooklabs.com