BellBrook’s Assay Validation Sprint is a focused, two-week project designed to establish assay conditions, controls, and technical confidence before screening, compound validation, or CRO transfer.
At the end of the Sprint, your team receives a clear assessment of assay feasibility, recommended starting conditions, identified risks, and a practical next-step plan.
The Sprint is designed to answer the technical questions that can delay or undermine a biochemical discovery program.
Determine whether the target and reaction can support a practical biochemical assay workflow.
Define an appropriate detection format, reagent strategy, controls, and starting protocol.
Understand whether the workflow is ready for screening, requires additional optimization, or should follow a different assay approach.
The goal is not simply to generate more data. It is to give your team enough confidence to make the next assay decision.
Every Assay Validation Sprint begins with a focused review of the target or enzyme class, reaction, available materials, current data, assay objective, and technical risks. The project is then scoped around the specific decision your team needs to make.
The written summary documents the recommended workflow, important assay risks, available supporting data, and whether the assay appears suitable for screening, compound testing, or transfer.
Depending on the assay, available materials, and project goal, the Sprint may also include focused experimental work.
Potential experimental modules include:
The experimental scope is defined before the project begins. Your proposal will identify the planned work, materials, deliverables, timeline, and fixed project price.
BellBrook develops and supports biochemical assay workflows across a wide range of drug discovery targets, including:
Your target does not need to appear in our existing catalog. BellBrook can evaluate whether one of our detection technologies or assay-development approaches is appropriate for the reaction.
BellBrook’s technologies directly measure enzyme-generated nucleotides and other reaction products without relying on multienzyme coupling cascades.
This can reduce workflow complexity and help identify issues that may be obscured by coupled detection systems.
Our scientists understand enzyme kinetics, assay-format selection, reagent requirements, control design, and the practical requirements of screening workflows.
BellBrook can provide the detection reagents, active enzyme, starting protocol, experimental validation, and implementation support needed to move the assay forward.
The result is a workflow that can remain with BellBrook, transfer to a CRO, or be implemented in your internal laboratory.
Tell us what you can about your assay objective, reaction, available materials, or current technical challenge.
You do not need to disclose your target during the initial review. You may provide only the enzyme class, reaction type, current assay format, or information you are comfortable sharing.
BellBrook will review the request and determine whether an Assay Validation Sprint is an appropriate next step. If it is a fit, we will provide a defined project scope, timeline, deliverables, and price before work begins.
What is an Assay Validation Sprint?
It is a focused scientific project designed to determine whether a biochemical assay workflow is feasible, define appropriate starting conditions and controls, identify technical risks, and recommend the best next step.
The Sprint can include scientific review, experimental validation, data analysis, protocol development, or implementation support depending on the agreed scope.
How long does the Sprint take?
Most projects are designed around a two-week technical effort after the required enzyme, substrates, compounds, and other materials are available.
The exact schedule depends on material availability and the experimental scope. The proposed timeline is confirmed before work begins.
Do we need to disclose our target?
No. A target name is not required for the initial feasibility review.
You may provide the enzyme class, reaction type, assay format, or technical challenge instead. BellBrook can also put an NDA in place before target-specific information is discussed.
Is this a CRO service?
The Sprint can complement your internal team or CRO.
BellBrook can define and validate the assay workflow, perform focused experimental work, prepare reagents and protocols, or support transfer to your selected CRO. The Sprint is not intended to replace a large screening campaign.
Do we need to use BellBrook reagents?
The Sprint is built around BellBrook’s biochemical assay technologies when they are appropriate for the reaction and project objective.
The initial feasibility review helps determine whether a BellBrook platform is a suitable fit before a project is proposed.
Can BellBrook test compounds?
Yes, when appropriate for the target and assay format.
A Sprint may include pilot compound testing, concentration-response analysis, orthogonal confirmation, or control-compound evaluation. The number of compounds and planned experiments are defined in the project scope.
Do you offer reagents?
Yes. BellBrook can provide custom reagents packages for your project. This includes enzyme, buffer, substrates, homogenous assay reagents, assay plates, protocols, and support.
Can BellBrook support our CRO?
Yes. BellBrook can provide the assay strategy, reagents, protocols, control recommendations, quality expectations, training, and troubleshooting support needed for CRO implementation.
What if we do not have an assay yet?
An existing assay is not required.
BellBrook can evaluate the enzyme reaction, available materials, project objective, and detection options to recommend a practical starting workflow.
How is pricing determined?
Pricing depends on the assay status, available materials, experimental work, number of compounds, and required deliverables.
After the feasibility review, BellBrook will provide a defined proposal describing the scope, timeline, deliverables, and fixed project price.
BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400
info@bellbrooklabs.com