CROs Are Being Asked to Do More With Harder Targets

CRO screening teams are increasingly asked to support complex enzyme targets, focused compound sets, mechanistic follow-up, and client programs where assay confidence matters as much as throughput. Standard workflows are useful, but they do not always provide the orthogonal biochemical evidence needed to advance the right compounds.

Microplate experiment data analysis workflow

Where BellBrook Fits

Orthogonal Hit Validation

Confirm hits from primary or AI screens using a direct biochemical readout.

Difficult Target Assay Development

Support assay setup for enzyme classes that may not fit routine workflows.

HTS-Ready Biochemical Screening

Move from assay setup to scalable screening conditions with practical technical support.

Direct Detection for Decision-Quality Data

BellBrook’s Transcreener assays directly detect nucleotide products such as ADP, AMP, GDP, UDP, cGAMP, ADPR, and pADPR. This gives CRO teams flexible biochemical readouts across many enzyme classes, including targets where coupled or indirect assay formats may create uncertainty.

Not every program requires a new assay format. But when a client needs orthogonal confirmation, difficult target support, or a direct biochemical readout, BellBrook can help.

Target Classes Supported
  • Kinases
  • ATPases
  • Helicases
  • GTPases
  • cGAS
  • PARPs
  • PARG
  • CD38
  • SARM1
  • SIRTs
  • CD39 / CD73
  • ENPP1
  • PDEs
  • Glycosyltransferases
  • Methyltransferases

Biochemical Assay Support for CRO Screening and Hit Validation

Learn how CRO teams can use orthogonal biochemical assays to support difficult target screening, improve hit confidence, and reduce assay risk in this 12-page guide.

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Work with BellBrook in the Way That Fits Your Program

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Assay Fit Review

Tell us the target class, biology, and assay stage. We’ll recommend a practical path forward.

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Kit-Based Evaluation

Use BellBrook assay reagents in your own workflow with setup guidance.

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Assay Development Support

Get help optimizing conditions, controls, signal window, and Z′ performance.

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BellBrook Services Support

When useful, BellBrook can support assay work directly through internal services.

A Strong Fit for Hit Validation and AI-Enabled Workflows

As more teams use virtual screening and AI-guided prioritization to narrow compound sets before testing, the need for reliable biochemical validation becomes even more important.

This video is explicitly focused on how biochemical assays are used after either biochemical HTS or virtual screening, with applications in hit confirmation, triaging, hit expansion, mechanism studies, and residence time analysis.

If your team is moving from in silico prioritization to experimental validation, an orthogonal biochemical assay can help you:

  • confirm active compounds quickly
  • remove false positives before deeper investment
  • generate stronger data for progression decisions
CRO Biochemical Assay Support

Help your CRO expand biochemical assay capabilities with HTS-ready detection technologies, optimized enzyme systems, and expert assay support from BellBrook Labs.

See What Other Scientists Are Saying

“The ADP assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.”

“We were struggling with a narrow assay window and poor sensitivity with luciferase-coupled ATPase assays. Initially, we had Bellbrook run their Transcreener ATPase assay in-house as a contracted project. We were so pleased with the results that we immediately onboarded the assay to our company lab. Throughout the whole process, Bellbrook scientists were clear, rapid communicators and demonstrated their deep knowledge of the assay. We highly recommend Bellbrook to our peers in drug discovery.”

“Excellent blend of simplicity & good science. Excellent technical support”

“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook knows what they are doing.”

“Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.”

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Trusted by Over 700+ Labs Globally for 20+ Years

Request a CRO Assay Fit Review

Why CROs Work With BellBrook Labs.

  • HTS-ready biochemical assays
  • Direct detection readouts
  • Broad enzyme class coverage
  • Support for difficult targets
  • Practical assay setup guidance
  • Scalable formats for screening
  • Cost-effective reagent supply
  • Scientist-to-scientist troubleshooting

We understand that confidentiality in this business is critical. Our goal is to accelerate your discovery by providing the best assay available, coupled with expert support. We can fast track an NDA if desired.

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