Find a Biochemical Assay for Your Enzyme Target

Browse assay approaches, products, and supporting data for enzyme targets across drug discovery. BellBrook’s target-specific solutions may include a validated detection method, active enzyme, complete Enzolution® Assay System, starting protocol, or scientific support to establish the assay in your laboratory.

Don’t See Your Target

The targets listed below represent assays BellBrook has developed, tested, or supported. They are not the limit of what our technologies can measure. Transcreener® assays directly detect nucleotide products such as ADP, AMP, GDP, UDP, cGAMP, ADPR, and others. AptaFluor® directly detects SAH produced by methyltransferase reactions. Because these platforms detect the reaction product rather than a specific enzyme, they can be adapted to many nucleotide-producing enzymes that are not currently listed.

Ask About an Unlisted Target

Browse Assays by Target Class

Select a target to view available assay products, performance data, protocols, complete assay systems, or related scientific resources.

Availability varies by target. Contact BellBrook for help identifying the most appropriate product or workflow for your program.

METHYLTRANSFERASES

STING-PATHWAY

ATPASES

NUCLEOTIDASES

EXONUCLEASES

GLYCOSYLTRANSFERASES

PHOSPHODIESTERASES

OAS

OAS1 Assay

PARP

ADPR Producing

Sirtuins

OTHER

Assay Solutions for Your Toughest Targets

BellBrook Labs offers cutting-edge assays to meet the demanding needs of drug discovery researchers. Leveraging our Transcreener and AptaFluor Assay platforms, our scientists continuously work to validate and develop assays ready for your most challenging enzyme targets.

Our assay systems include the enzyme, substrate, assay plates, and buffers required for the respective Transcreener or AptaFluor Assay, providing a complete assay solution to simplify your discovery process.

Cut your development time from months to days!

Transcreener Assay Technology

Transcreener technology uses a simple but powerful method that consists of antibodies selective to nucleotides and far-red fluorescent tracers. The product produced in the reaction competes with the tracer, changing the fluorescent properties and providing a readout.

Transcreener is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The system yields robust results that also make it extremely amiable to HTS.

AptaFluor Assay Technology

AptaFluor SAH Methylatransferase Assay Schematic

The AptaFluor SAH Methyltransferase Assay uses a naturally occurring aptamer that selectively binds SAH, the product of methyltransferase reactions.

The exquisite affinity and selectivity of the aptamer combined with a positive TR-FRET signal enable screening and profiling of methyltransferases with unparalleled sensitivity.

As a result, the AptaFluor SAH Methyltransferase Assay overcomes technical gaps of current detection methods that are not sensitive enough for enzymes with low turnover or high SAM affinities.

Enzolution™ Assay Systems

General Assay System Graphic Without Transcreener Assay Depiction

Our Enzolution Assay Systems provide a comprehensive approach to assess enzyme activity and screen for novel modulators. It includes enzyme, substrate, buffers, and assay plates required for an enzyme reaction and screening.

Choosing to use our Enzolution Assay System with the Transcreener or AptaFluor Assay saves you time and money in sourcing enzymes and developing the assays.

Easy-to-Use & HTS-Ready

A simple assay protocol that is ready to scale up for HTS. (Z’ > 0.7)

Direct Nucleotide Detection

Many of our assays offer direct nucleotide detection, which reduces the risk of false positives or missing a hit.

Ultra-Sensitive Assay Technology

The unparalleled sensitivity of the assay technology allows high-throughput screening with reduced enzyme usage.

Outstanding Assay Reagent Stability Symbol

Outstanding Reagent Stability

Stability at room temperature for at least 8 hours ensures data quality does not suffer in a large, automated screen where detection mix may be stored for long periods at room temperature.

Resources

Find the right resources to help you get to your solution faster.

FAQ

Protocols

Publications

Instrument Compatibility

Application Notes

Posters

Contact a BellBrook Scientist Today

Interested in purchasing an assay or advancing your program with our drug discovery services? Have questions about the assay technology? Contact a BellBrook scientist today to see how we can help accelerate your discovery.

BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400

info@bellbrooklabs.com