Active, human DNA polymerase theta enzyme (AA 67-894), C-terminal 6xHis-tagged protein expressed and purified from insect cells. The enzyme has been thoroughly validated with the Transcreener ADP Assay Kit. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >90% in a composition of 20 mM HEPES, 5 mM NaCl, 5% Glycerol, and 0.5 mM TCEP (pH 7.5). Currently used with the Transcreener assay at a concentration of 0.42 nM in a 10 μL reaction.
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Alternate Names
- DNA polymerase theta
- POLH
- PRO0327
- Polymerase (DNA) theta
- DNA polymerase θ
Storage Information
- Store at -80ºC.
- Avoid freeze-thaws.
- Stable for up to 6 months from date of receipt.
