Recombinant eIF4A1 Enzyme, Human, Full-Length

Active, human, full-length eIF4A1 protein expressed and purified from E.coli. The enzyme has been thoroughly validated with the Transcreener ADP Assay Kit. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >90% in a composition of 50 mM Tris-HCl, 500 mM NaCl, and 10% Glycerol (pH 8.0).

Learn how eIF4A1 enzyme was used for screening and profiling with Transcreener.

Custom Sizes and Bulk Pricing Available Contact Us for an HTS-scale Quote!

$398.00

SKU: 2383 Category: Recombinant Enzymes

Alternate Names

Eukaryotic Translation Initiation Factor 4A1
DDX2A
EIF-4A
eIF-4A-I
eIF4A-I

Storage Information

Store at -80ºC.
Avoid freeze-thaws.
Stable for up to 6 months from date of receipt.

Recombinant eIF4A1 Enzyme

Certificates of Analysis

Cat. No. 2383 – 25 μg eIF4A1

C of A Lot# - 15152A

Active eIF4A1 Enzyme – Ready for Inhibitor Discovery

Here, we use 30 µM ATP with 1 mg/mL Yeast RNA substrate. The enzyme buffer included 50 mM Tris-HCl (pH 7.5), 0.25 mM MgCl₂, 1 mM TCEP, and 0.01% Triton. The enzyme reaction took place for 120 minutes at 30°C. The ADP Detection Mix included 1X Stop & Detect Buffer B, 4 nM ADP² Alexa Fluor 633 Tracer, and 33.4 µg/mL ADP antibody. The Detection Mix was added and incubated for 60 minutes at room temperature and read on a plate reader.

Activity may vary from lot to lot. Please see C of A for enzyme activity.

eIF4A1 Enzyme Titration

Linear Titration

Poster: The DNA Damage Response and Innate Immunity

Resources

Find the right resources to help you get to your solution faster.

FAQ

Protocols

Publications

Instrument Compatibility

Application Notes

Posters

Contact Us to Learn More

Please fill out the form below. We will respond quickly to get the conversation moving and learn how we can help. We keep things discrete, confidential, and professional.

“Some CROs reserve their best teams for high profile customers. If you’re not right there with them you get less experienced teams… BellBrook understands enzyme kinetics, so it made that part of the project so much easier. The larger CRO didn’t seem to have the expertise we needed in this area.”

- BioPharma Lead Discovery Program Manager

“The ADP assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.”

- Pharmaceutical Research Scientist

“Excellent blend of simplicity & good science. Excellent technical support”

- Director Pharma Drug Discovery Center

“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook Know what they are doing.”

- Biotech Program Manager

“The Transcreener AMP/GMP Assay is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay I needed 1 µM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with the assay development. Their representative has been very willing to quickly help me get my assay up and running.”

- University Research Scientist

“Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.”

- Biotech Research Scientist

Previous Next

BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400

info@bellbrooklabs.com