Recombinant eIF4A3 Enzyme, Human, Full-Length

Active, human, full-length eIF4A3 protein expressed and purified from E.coli. The enzyme has been thoroughly validated with the Transcreener ADP Assay Kit. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >85% in a composition of 50 mM Tris-HCl, 150 mM NaCl, 0.5 M L-Arginine, and 10% Glycerol (pH 8.0).

Learn how eIF4A3 enzyme was used for screening and profiling with Transcreener.

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$489.00

SKU: 2385 Category: Recombinant Enzymes

Alternate Names

Eukaryotic initiation factor 4A-III
ATP-dependent RNA helicase DDX48
ATP-dependent RNA helicase eIF4A-3
DEAD box protein 48
Eukaryotic translation initiation factor 4A isoform 3
DDX48

Storage Information

Store at -80ºC.
Avoid freeze-thaws.
Stable for up to 6 months from date of receipt.

Recombinant eIF4A3 Enzyme

Certificates of Analysis

Cat. No. 2385 – 50 μg eIF4A3

C of A Lot# - 15153A

Active eIF4A3 Enzyme – Ready for Inhibitor Discovery

Here, we use 30 µM ATP with 50 µg/mL Yeast RNA substrate. The enzyme buffer included 50 mM Tris-HCl (pH 7.5), 0.25 mM MgCl₂, 1 mM TCEP, and 0.01% Triton. The enzyme reaction took place for 120 minutes at 30°C. The ADP Detection Mix included 1X Stop & Detect Buffer B, 4 nM ADP² Alexa Fluor 633 Tracer, and 33.4 µg/mL ADP antibody. The Detection Mix was added and incubated for 60 minutes at room temperature and read on a plate reader.

Activity may vary from lot to lot. Please see C of A for enzyme activity.

eIF4A3 Enzyme Titration

Linear Titration

Poster: The DNA Damage Response and Innate Immunity

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“Some CROs reserve their best teams for high profile customers. If you’re not right there with them you get less experienced teams… BellBrook understands enzyme kinetics, so it made that part of the project so much easier. The larger CRO didn’t seem to have the expertise we needed in this area.”

- BioPharma Lead Discovery Program Manager

“The ADP assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.”

- Pharmaceutical Research Scientist

“Excellent blend of simplicity & good science. Excellent technical support”

- Director Pharma Drug Discovery Center

“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook Know what they are doing.”

- Biotech Program Manager

“The Transcreener AMP/GMP Assay is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay I needed 1 µM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with the assay development. Their representative has been very willing to quickly help me get my assay up and running.”

- University Research Scientist

“Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.”

- Biotech Research Scientist

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