Recombinant IDH1 Enzyme, Human

Active, human IDH1 wild-type enzyme, full-length, N-terminal 6xHis-tagged protein expressed and purified from E.Coli. The enzyme has been thoroughly validated with the Hydriscreener NADPH Assay Kits. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >95% in a composition of 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 20% glycerol, 1 mM TCEP. Currently, IDH1 enzyme is used with the Hydriscreener assay at a concentration of 500 pM in a 10 μL reaction.

Learn how IDH1 enzyme was used for screening and profiling with Hydriscreener.

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Alternate Names

IDH
WT IDH1
IDH1-wildtype
Isocitrate dehydrogenase [NADP] cytoplasmic, wild-type
Isocitrate dehydrogenase 1 (NADP+) 1

Storage Information

Store at -80ºC.
Avoid freeze-thaws.
Stable for up to 6 months from date of receipt.

Certificates of Analysis

Cat. No. 2395 – 5 μg IDH1

C of A Lot# 15234

Cat. No. 2396 – 50 μg IDH1

C of A Lot# 15234

Active IDH1 Enzyme – Ready for Inhibitor Discovery

Name: Recombinant IDH1 Enzyme, Human
SKU: 31625
Availability: InStock

The assay demonstrates linearity when raw data is converted to NADPH using a standard curve. We used 50 mM Tris pH 8.0, 5 mM MgCl₂, and 0.01% Triton X-100 for the buffer, and ran with 250 µM Isocitrate, and 75 µM NADP at 30°C for 1 hour. The Detection Mix was added and incubated at room temperature for 60 minutes and read with a CLARIOstar plate reader.

Activity may vary from lot to lot. Please see C of A for enzyme activity.

Enzyme Titration

Linear Response

Hydriscreener NADPH Assay IDH1 Wildtype Enzyme Linear Titration

Application Note: Detection of IDH1 Dehydrogenase Activity with the Hydriscreener™ NADPH Assay

Download the Application Note

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“Some CROs reserve their best teams for high profile customers. If you’re not right there with them you get less experienced teams… BellBrook understands enzyme kinetics, so it made that part of the project so much easier. The larger CRO didn’t seem to have the expertise we needed in this area.”

- BioPharma Lead Discovery Program Manager

“The ADP assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.”

- Pharmaceutical Research Scientist

“Excellent blend of simplicity & good science. Excellent technical support”

- Director Pharma Drug Discovery Center

“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook Know what they are doing.”

- Biotech Program Manager

“The Transcreener AMP/GMP Assay is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay I needed 1 µM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with the assay development. Their representative has been very willing to quickly help me get my assay up and running.”

- University Research Scientist

“Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.”

- Biotech Research Scientist

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BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400

info@bellbrooklabs.com

Also required (not included):

Hydriscreener™ NADPH Assay Kits for Dehydrogenases