Recombinant PARP2 Enzyme, Human

Active, full-length human PARP2 (AA 1-583) protein expressed and purified from Baculovirus infected insect cells with an N-Terminal His tag. The enzyme has been thoroughly validated with the Transcreener pADPr Assay Kit. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >90% in a composition of 50 mM TRIS, 500 mM NaCl, and 5% Glycerol (pH 8.0). Currently used with the Transcreener assay at a concentration of 3 nM in a 10 μL reaction.

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Alternate Names

Poly(ADP-ribose) polymerase 2
NAD+ ADP-ribosyltransferase 2
poly(ADP-ribose) synthase 2
ADPRT2

Storage Information

Store at -80ºC.
Avoid freeze-thaws.
Stable for up to 6 months from date of receipt.

Recombinant PARP2 Enzyme

Name: Recombinant PARP2 Enzyme, Human
SKU: 27527
Availability: InStock

Certificates of Analysis

Cat. No. 2347 – 5 μg PARP2 Enzyme

C of A - Lot# 14942

Cat. No. 2348 – 50 μg PARP2 Enzyme

C of A Lot# 14942

Active PARP2 Enzyme – Ready for Inhibitor Discovery

Here, we use 100 µM NAD with 0.25 mg/mL sheared salmon sperm DNA and 1X coupling enzyme mix. The enzyme buffer included 50 mM Tris-HCl (pH 8.0), 5 mM MgCl₂, 100 mM NaCl, and 0.01% Triton X-100. The enzyme reaction took place for 60 minutes at 30°C. The AMP Detection Mix included 1X Stop & Detect Buffer B, 8 nM ADP² Alexa Fluor 633 Tracer, and 36 µg/mL AMP antibody. The Detection Mix was added, incubated for 120 minutes at room temperature, and read on a plate reader.

Activity may vary from lot to lot. Please see C of A for enzyme activity.

PARP2 Enzyme Titration

Enzolution PARP2 Assay Enzyme Titration FP Readout

Linear Response

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“Some CROs reserve their best teams for high profile customers. If you’re not right there with them you get less experienced teams… BellBrook understands enzyme kinetics, so it made that part of the project so much easier. The larger CRO didn’t seem to have the expertise we needed in this area.”

- BioPharma Lead Discovery Program Manager

“The ADP assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.”

- Pharmaceutical Research Scientist

“Excellent blend of simplicity & good science. Excellent technical support”

- Director Pharma Drug Discovery Center

“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook Know what they are doing.”

- Biotech Program Manager

“The Transcreener AMP/GMP Assay is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay I needed 1 µM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with the assay development. Their representative has been very willing to quickly help me get my assay up and running.”

- University Research Scientist

“Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.”

- Biotech Research Scientist

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