BellBrook’s drug discovery services specialize in small molecule modulators for enzyme drug targets. We have conducted assay development & lead discovery for over 15 years. Our experts work diligently with varieties of customers in early-stage drug discovery programs. From assay development to lead discovery, we provide quality data to accelerate programs.
BellBrook scientists performed a detection of purified, full-length human cGAS with the Transcreener cGAMP FP Assay. Linear response is shown from polarization data converted to cGAMP using a standard curve.
BellBrook scientists performed an inhibitor screening & inhibitor potency profiling of cGAS using the Transcreener cGAMP cGAS Assay.
Shown above is a screen of 1600 compounds. cGAS was used at 10 nM, compounds were at 10 μM. Z = 0.62, Z’ = 0.7 illustrate a robust screening assay. Hits were determined to be anything outside three standard deviations from the mean.
Confirmation of a screening hit using the Transcreener cGAMP cGAS Assay in the FP format. Follow-up assays to triage screening hits can be used to select bona fide inhibitors for advancement into medicinal chemistry/SAR.
BellBrook’s Transcreener® HTS assay platform is ideally suited for the detailed biochemical and kinetic analyses required for hit-to-lead programs. Transcreener® is an extensively validated HTS platform that produces robust FP, TR-FRET, and FI signals for nucleotides produced by enzymes; e.g. ADP for kinases. It has been used to screen tens of millions of wells over the last 10 years in pharma and biotech labs.
Because they use direct immunodetection of nucleotides, Transcreener® assays can provide more reliable results than alternative methods that rely on complicated assay schemes. In addition, the ability to run Transcreener® assays in continuous mode enables analysis of inhibitor binding kinetics, such as residence time, which is increasingly recognized as an important factor for drug efficacy. For our services, additional assay methods will be employed as needed to meet customer requirements.
Leveraging BellBrook’s services expertise will save you time and money. Your project will be handled by the same BellBrook scientists who developed the Transcreener assay and have optimized them for more than 100 different enzymes, including kinases, ATPases, GTPases, glycosyltransferases, ligases/synthetases, and phosphodiesterases. We will tailor our efforts to meet your specific needs. For example, minimizing consumption of limited reagents or enabling simultaneous screening for inhibitors and activators. And we will work fast. If your target enzyme is in good shape, we can generally complete the services within two to four weeks.
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What services does BellBrook Labs provide?
BellBrook delivers a comprehensive suite of drug discovery services driven by decades of enzymology experience:
Biochemical Assay Development Services
Lead Discovery Services
Specialized profiling offerings: ATPase, GTPase, Helicase, cGAS/STING, and Thermal Shift Assays
What is your scientific approach and technology platform?
We specialize in small-molecule modulators for enzyme drug targets, with nearly two decades of experience in assay development and lead discovery.
Our core detection platform is Transcreener® HTS technology, recognized for robust detection of nucleotides (e.g., ADP, GDP) via FP, FI, or TR-FRET, with high reproducibility and minimal compound interference.
What’s your general process from project start to delivery?
Choose Your Target – Even if your target isn’t listed, we likely have experience or can develop a tailored solution.
Consult with a Scientist – Discuss your goals, timelines, and dataset needs in detail.
We Execute the Work – In our Madison, WI lab, we develop HTS-ready assays using proven Transcreener techniques.
Report & Support – You’ll receive raw data, analysis, and ready-to-use HTS/hit-to-lead protocols.
What specific profiling services are available?
Assay Development – Includes target protein handling, optimization of buffers and conditions, and validation through dose-response, kinetic, or interference screens.
Lead Discovery – Services such as inhibitor screening, potency and selectivity profiling, residence time measurement, mechanism-of-action studies, triage of non-stoichiometric inhibitors, and compound-binding verification via thermal shift assays.
Target-Specific Profiling – Including ATPase, GTPase, helicase, and cGAS/STING profiling—each using tailored assay formats and real-time collaboration.
Why should clients prefer BellBrook over other providers?
Expertise in Enzymology – Our team includes the very scientists who developed Transcreener technology, optimizing assays for over 100 enzyme targets.
Speed – Rapid project turnaround—many assays are completed within 2 to 4 weeks if enzymes are well-prepared.
Proven Platform – Transcreener is a mature, widely-adopted HTS platform, trusted across pharma and biotech.
Customized Service – Tailored workflows, minimized reagent usage, and direct scientific collaboration ensure efficiency and precision.
Real-World Testimonials – Clients highlight reproducibility, technical support, and quick turnaround as standout strengths
What targets and enzyme families have you worked with?
BellBrook supports a broad range of enzyme classes, including:
Protein kinases, lipid/metabolic kinases
Acetyltransferases, HDACs
GTPases/GAPs/GEFs
ATPases, phosphodiesterases, helicases
Nucleotidases, ligases/synthetases, glycosyltransferases
What makes Transcreener assays stand out?
Mix-and-Read Format – Simple, homogeneous assays without complex coupling steps.
Direct Nucleotide Detection – Reduces interference and improves data clarity.
Broad Applicability – Robust detection across thousands of enzyme targets with FP, FI, and TR-FRET formats.
Continuous/Kinetic Capability – Supports analysis like residence time that’s critical for pharmacological relevance.
BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400
info@bellbrooklabs.com
