BellBrook’s Scientists Use the Proven Transcreener GDP Assay to Directly Measure the Activity of GTPase Enzymes as Well As GAPs and GEFs to Get You Accurate Results Fast

Interested in moving your program forward, but don’t want to bring an assay in-house? Our scientists can help! BellBrook scientists right here in Madison, Wisconsin will use their extensive biochemistry and enzymology expertise to work with you and accelerate your GTPase program. We can provide inhibitor potency profiling for a variety of GTPase enzymes and related proteins. Get accurate IC50 results fast, to understand how your inhibitor interacts with other GTPases. Contact us today to see if BellBrooks’s GTPase profiling services will advance your research.

Standard GTPase Profiling Menu

Choose from this menu or inquire about other GTPases, GAPs, and GEFs.

GTPase FamilyEnzymeEnzyme Type
Ras FamilyKRasWild Type
KRas G12CMutant
KRas G12DMutant
KRas G12VMutant
HRasWild Type
HRas G12CMutant
NRasWild Type
NRas G12CMutant
RRasWild Type
Rho FamilyRhoAWild Type
RhoCWild Type
Cdc42Wild Type
Rac1Wild Type
Rab FamilyRab2Wild Type

How It Works

GTPase Enzyme Reaction

Gap activity assay on off cycle

Measure Activity with the Transcreener GDP Assay

GTPase Profiling Services Assay Schematic

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Related Resources

  1. Profiling GTPase Inhibitors for Effects on GTP Hydrolysis Using the Transcreener GDP Assay (FASEB 2024)
  2. A Homogenous, High-Throughput Assay to Measure Guanine Nucleotide Exchange Factor Activity Using Transcreener GDP Assay (SLAS 2017)
  3. Interrogation of Modulators of GPCR and small GTPase Activity: Screening GAPs and GEFs with the Transcreener GDP Assay (DOT 2015)
  4. Selective Detection of LRRK2 GTPase Activity with the Transcreener GDP Assay (SLAS 2013)
  5. Investigation of different GTPase classes with the Transcreener GDP Assay (SLAS 2012)
  6. Discovery of small molecule inhibitors for RGS proteins using the RGS screen Platform (SBS 2011)
  7. Targeting RGS Proteins using Engineered Galpha Proteins and the Transcreener GDP Assay (SBS 2010)
  8. Engineering G alpha Proteins for Detection of RGS Protein GAP Activity with the Transcreener GDP Assay (Screening Europe 2009)
  9. GDP Detection Using the Transcreener HTS Assay Technology (SBS 2008)
  10. An RGS Protein HTS Strategy using the Transcreener GDP Assay (SBS 2008)

Journal of Biological Chemistry. 2019 Nov 8;294(45):16865-83.
L-type prostaglandin D synthase regulates the trafficking of the PGD2 DP1 receptor by interacting with the GTPase Rab4.
Binda C, Génier S, Degrandmaison J, Picard S, Fréchette L, Jean S, Marsault E, Parent JL.

Methods in Molecular Biology, 2018. vol 1821. Humana Press, New York, NY
High-Throughput Assay for RhoGEFs Based on the Transcreener® GDP Assay. In: Rivero F. (eds) Rho GTPases.
Lowery R.G., Kumar M.

PloS one. 2017 Sep 28;12(9):e0185639.
A highly-sensitive high throughput assay for dynamin’s basal GTPase activity.
Mohanakrishnan A, Tran TV, Kumar M, Chen H, Posner BA, Schmid SL.

Journal of Virology. 2015 Nov 1;89(21):10879-90.
Functional comparison of Mx1 from two different mouse species reveals the involvement of loop L4 in the antiviral activity against influenza A viruses.
Verhelst J, Spitaels J, Nürnberger C, De Vlieger D, Ysenbaert T, Staeheli P, Fiers W, Saelens X.

J Biomol Screen. 2015 Jul 20. pii: 1087057115596326. [Epub ahead of print]
A High-Throughput Assay for Rho Guanine Nucleotide Exchange Factors Based on the Transcreener GDP Assay
Reichman M, Schabdach A, Kumar M, Zielinski T, Donover PS, Laury-Kleintop LD, Lowery RG.

Curr Protoc Pharmacol. 2012 Mar;Chapter 2:Unit2.8.
Evaluating Modulators of “Regulator of G-protein Signaling” (RGS) Proteins.
Bosch DE, Zielinski T, Lowery RG, Siderovski DP.

J Biomol Screen. 2011 Aug;16(7):717-23. Epub 2011 May 18
High-throughput fluorescence polarization assay for the enzymatic activity of GTPase-activating protein of ADP-ribosylation factor (ARFGAP).
Sun W, Vanhooke JL, Sondek J, Zhang Q.

J Biomol Screen. 2009.
Two Gαi1 Rate-Modifying Mutations Act in Concert to Allow Receptor-Independent, Steady-State Measurements of RGS Protein Activity
Zielinski T, Kimple AJ, Hutsell SQ, Koeff MD, Siderovski DP, Lowery RG.

  1. Researchers from BellBrook Labs Pen Chapter in Springer’s Rho GTPases: Methods and Protocols
  2. Researchers Employ GTPase Assay to Investigate Rab Proteins

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Resources

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“The assay itself has good reproducibility and provides a clear readout on our enzyme activity. The most important piece, however, is the level of attention to our project and rapid turnaround time. The staff at BellBrook Know what they are doing.”

“The Transcreener AMP/GMP Assay is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay I needed 1 µM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with the assay development. Their representative has been very willing to quickly help me get my assay up and running.”

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BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400

info@bellbrooklabs.com