Complete Biochemical Assay Solutions for Your Toughest Enzyme Targets
BellBrook Labs Offers Complete Biochemical Assay Solutions for Your Toughest Enzyme Targets. Whether you are screening a million compounds, prioritizing hits from a virtual screen, or optimizing a lead molecule, our enzyme activity assay solutions will generate the high-quality data you need to advance your program. Learn more about what we do to accelerate discovery.
Why Does Sensitivity Matter for HTS Assays?
Sensitivity is critical to HTS assay development. The two biggest reasons include using less enzyme and allowing for flexible assay conditions. Using less enzyme saves the user a lot of money when screening. Transcreener assays are up to ten times more sensitive than competitor assays. Learn more about why assay sensitivity is critical to your HTS campaign.
How Do Transcreener HTS Biochemical Assays Work?
Transcreener is an HTS assay platform for measuring enzyme activity. In this video, we use kinase activity assays as an example. A kinase uses ATP as a phosphate donor, leaving ADP as a product. By measuring a common product of enzyme reactions such as ADP, the assay is universal, meaning it can be used to measure the activity of any enzyme that produces that product. Learn more about how Transcreener works, including its three fluorescent readouts.
Advantages of cGAMP Detection vs ATP Depletion Assays
Cyclic GAMP synthase, or cGAS, is a compelling therapeutic target for intractable autoimmune diseases like lupus, irritable bowel disease, and rheumatoid arthritis. This video describes how the Transcreener cGAMP cGAS Assay can help researchers unlock novel cGAS inhibitors. The advantages of cGAMP detection over ATP depletion are described.
Advantages of GDP Detection Over GTP Depletion Assays for GTPase Discovery
Exciting new strategies for inhibiting GTPases in the Ras and Rho families have emerged over the last several years. This video describes how the Transcreener GDP GTPase Assay can help researchers unlock novel GTPase inhibitors. The advantages of GDP detection over GTP depletion assay strategies are described.
Finding the Right Biochemical Assay for HTS and Lead Discovery
Finding the right enzyme activity assay is crucial for drug discovery. Transcreener is ultra-sensitive, simple, robust, and accurate, making it easily scalable for high-throughput screening (HTS) and flexible enough to run in kinetic mode for streamlined assay development and SAR studies.
How Enzolution Assay Systems Can Accelerate Your Inhibitor Discovery
Enzolution assay systems are the solution, saving you time and resources when launching your inhibitor screening campaign. Enzolution assay systems include validated enzyme, substrates, assay buffer, plates, and protocols required to run an enzyme activity assay in an HTS format. We’ve done the work so you don’t have to saving you months of validation work!
Why Z Prime is More Accurate for Measuring Assay Performance Than Signal to Background Ratios
This video explores the art of effectively comparing different assays by honing in on the right performance metrics. By mastering these metrics, you can elevate your evaluations, whether you’re navigating the realms of research or industry.
From Virtual Hits to Validated Inhibitors: How to Confirm Virtual Screening Results
Virtual screening is a powerful approach in small-molecule drug discovery. But identifying a hit is just the beginning. To move beyond predictions, you need biochemical validation. In this video, we walk through the critical steps for confirming virtual screening hits.
Breakthrough in Methyltransferase Assays: Using the AptaFluor SAH Assay for HTS
In this video, we introduce the AptaFluor® SAH Assay: a next-generation solution for detecting methyltransferase activity with unmatched sensitivity and specificity. Our team developed a TR-FRET-based, homogeneous assay capable of detecting low nanomolar SAH in the presence of excess SAM—meeting the stringent requirements of even the slowest MT enzymes.
Heliscreener RNA Unwinding Assay for HTS Drug Discovery
Discover the Heliscreener RNA Unwinding Assay—a high-throughput, fluorescence-based assay for DEAD-box helicases. Ideal for drug discovery, inhibitor screening, and RNA metabolism research.
Accelerate Drug Discovery with BellBrook Assay Development and Hit-to-Lead Services
Accelerate drug discovery without adding headcount. In today’s lean biotech environment, timelines are tight and budgets tighter—yet the pressure to move faster never stops. BellBrook Labs plugs into your workflow with assay development, screening, and hit-to-lead services built for speed, scientific rigor, and clear go/no-go decisions.
Overcoming SIRT Assay Limitations: Fast, Homogeneous Detection of OAADPr
In this video, we introduce a new universal Sirtuin activity assay that measures OAADPr in real time by enzymatically converting it to AMP, which is detected using the Transcreener AMP Assay with either an FP or TR-FRET readout. This innovative method eliminates the complexity of substrate-specific readouts and enables reliable, quantitative measurement of SIRT enzymatic activity for any substrate and any Sirtuin.
High-Throughput PARP Inhibitor Screening with a Mix-and-Read Biochemical Assay
This video highlights a high-throughput, mix-and-read biochemical assay for quantitative PARP activity and inhibitor screening. Using enzymatic conversion of poly(ADP-ribose) to AMP with Transcreener® detection, the assay delivers exceptional sensitivity, automation compatibility, and accurate IC₅₀ determination for PARP drug discovery.
XRN1 Assays for HTS and Hit-to-Lead
A high-throughput XRN1 assay using Transcreener® technology enables direct detection of nucleotide monophosphates from RNA degradation. Compatible with diverse RNA substrates and validated for HTS, it provides a simple, scalable, and physiologically relevant solution for screening XRN1 inhibitors.
Getting Started with Transcreener – A Quick Start Guide
Set up Transcreener assays with confidence. This quick-start video covers four key steps for robust HTS-ready performance: choosing the right plate, configuring your plate reader, matching the detection mix to your substrate concentration, and optimizing enzyme concentration for reliable screening and inhibitor profiling data.
CRO Biochemical Assay Support
Help your CRO expand biochemical assay capabilities with HTS-ready detection technologies, optimized enzyme systems, and expert assay support from BellBrook Labs.
