TL;DR — Key Takeaways for HTS Teams Many kinase screening failures are assay design failures, not compound failures. Poor Z′, narrow signal windows, and unstable enzymes almost always trace back to ATP, substrate, or format misalignment. Many common artifacts (ATP competition effects, compound interference, edge effects) are predictable and preventable. Endpoint assays—especially when over-coupled or […]
Quick Answer Developing an HTS-ready biochemical assay typically takes 6–16 weeks, depending on target novelty, reagent availability, detection format, and required robustness. Well-characterized enzymes using off-the-shelf reagents can be ready in as little as 4–6 weeks, while novel targets or custom reagents often require 3–4 months or more. Most delays come from enzyme supply, assay […]
TL;DR Biochemical HTS assays measure the activity of a purified target (such as an enzyme) in a controlled, cell-free system, while cell-based HTS assays measure compound effects inside living cells. Biochemical assays offer higher control, reproducibility, and throughput, whereas cell-based assays provide greater biological context. In practice, both are complementary and are often used at […]
Summary To validate an assay for high-throughput screening (HTS), you systematically test whether it produces robust, reproducible, and interference-resistant data at scale. This typically includes plate uniformity, dynamic range, Z′ factor, signal stability, reproducibility, DMSO tolerance, edge effects, and interference checks—performed under conditions that match how the assay will actually be screened. Why assay validation […]
TL;DR Successful HTS campaigns are built upstream—assay design decisions determine data quality, false-positive rates, and total program cost. Direct-detection biochemical assays (including fluorescence-based formats) are fully HTS-ready and can be equally or more sensitive than luminescent assays depending on design. A robust workflow moves deliberately from target biology → assay format → reagent selection → […]
A practical, HTS-first decision guide for enzymatic assays in drug discovery (with Transcreener® readout selection) TL;DR (Quick answer) Selecting a fluorescent readout is less about “what’s brightest” and more about what stays accurate and reproducible when you scale to real screening conditions (large compound libraries, higher compound concentrations, miniaturization, automation, and messy matrices). Fluorescence Intensity […]
TL;DR Best general HTS approach (for UDP-sugar glycosyltransferases): detect UDP product using a homogeneous, mix-and-read method. Classic options: radiometric assays (sensitive but operationally heavy), MS/HPLC (information-rich but lower throughput), and coupled-enzyme/luminescent assays (HTS-friendly but can add interference risk). Transcreener® UDP² Glycosyltransferase Assay directly measures UDP formation via competitive immunodetection and supports FP, FI, and TR-FRET […]
Why fluorescence remains the most versatile detection strategy for HTS—and why Transcreener’s three far-red fluorescent readouts are uniquely suited for real-world screening. TL;DR Fluorescent enzymatic assays are widely used in drug discovery because they support homogeneous workflows, high-throughput screening (HTS), assay miniaturization, and flexible detection formats. While all detection technologies carry some risk of assay […]
TL;DR — HTS-Ready Screening for SIRT3 Modulators SIRT3 is a mitochondrial NAD⁺-dependent deacetylase and a high-value drug discovery target, but it can be challenging to screen at scale. An HTS-ready approach combines optimized biochemical assay conditions with a homogeneous, mix-and-read detection format that directly reflects enzyme activity. Using Enzolution™ SIRT3 Assay Systems together with Transcreener® […]
Quick Answer Enzyme assay consumable subscription services provide laboratories with recurring, pre-planned deliveries of biochemical assay reagents—such as detection kits, enzymes, buffers, and controls—used for drug discovery, high-throughput screening (HTS), and routine enzymology. They are designed to reduce procurement friction, prevent reagent stockouts, improve assay consistency, and help teams scale screening programs without constantly re-ordering […]
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