Summary A reliable kinase assay must provide accurate, reproducible, and interference-resistant measurement of kinase activity under high-throughput conditions. The most dependable kinase screening assays—like the Transcreener® ADP² Kinase Assay—use direct detection of the enzymatic product (ADP) to ensure broad target compatibility, superior signal stability, and consistent performance across inhibitor screens. The Importance of Kinases in […]
Optimizing sensitivity, specificity, and throughput in cGAS/STING pathway research. Quick Answer Accurate measurement of cGAMP is essential for evaluating cGAS activity and screening modulators of the STING pathway. The Transcreener® cGAMP Assay from BellBrook Labs provides a direct, homogeneous, high-throughput method to measure cGAMP without labels or complex extraction steps—offering sensitivity, robustness, and reproducibility for […]
Summary Optimizing assay performance is essential for obtaining reliable, reproducible, and cost-effective results in high-throughput screening (HTS). Factors such as signal window, Z’-factor, substrate concentration, reagent stability, and plate uniformity determine the success of screening campaigns. At BellBrook Labs, our Transcreener® biochemical assays simplify optimization by using direct detection of universal assay products—like ADP, AMP, […]
Summary GTPases act as molecular switches that regulate signaling, transport, and cytoskeletal control by cycling between GTP-bound (active) and GDP-bound (inactive) states. Screening for inhibitors or modulators of GTPases requires accurate measurement of GTP hydrolysis. BellBrook Labs’ Transcreener® GDP GTPase Assay directly detects GDP produced by GTPases, enabling homogeneous, mix-and-read high-throughput screening (HTS) across validated […]
Summary False positives are a common problem in ADP detection assays used to measure kinase, ATPase, or other ATP-dependent enzyme activity. These misleading signals can derail high-throughput screening (HTS) campaigns and waste valuable time and resources. The best way to minimize false positives is to use a direct detection method, such as the Transcreener® ADP² […]
Summary An inhibitor screening assay identifies compounds that block enzyme activity — a crucial step in small-molecule drug discovery. Universal platforms such as BellBrook Labs’ Transcreener® Assays provide mix-and-read detection of nucleotide products (ADP, AMP, GDP, UDP, etc.) that can be applied to many enzyme classes. This single assay format accelerates assay development, minimizes false […]
Quick Answer The best glycosyltransferase assay for high-throughput screening (HTS) is one that is sensitive, universal, and homogeneous, allowing researchers to detect activity across many enzymes and substrates with minimal interference. The Transcreener® UDP Glycosyltransferase Assay from BellBrook Labs stands out as the most reliable and broadly applicable solution, offering direct detection of UDP, the […]
Quick Answer The best helicase enzyme assay combines high sensitivity, robustness, and throughput while maintaining biological relevance. In most drug discovery workflows, fluorescence-based strand displacement – such as the Heliscreener Platform or ADP detection assays—such as the Transcreener® ADP² platform—offer the optimal balance. These homogeneous “mix-and-read” assays deliver high Z′ factors and low false-positive rates, […]
Quick Answer The best PARP assay for screening and profiling is one that combines universality, high throughput, and robust detection of enzymatic activity across diverse PARP family members. The Transcreener® pADPr PARP Assay achieves this through direct measurement of poly-ADP-ribose (pADPr) produced by PARP enzymes—offering a homogeneous, fluorescence-based format with exceptional sensitivity, no coupling enzymes, […]
Poly(ADP-ribose) polymerases (PARPs) are central to DNA damage repair, and selective inhibition has emerged as a powerful synthetic lethality strategy. PARP inhibitors have already transformed the treatment of BRCA1/2-mutant cancers and continue to expand into breast, ovarian, prostate, and pancreatic indications. To accelerate the discovery of next-generation PARP inhibitors, BellBrook Labs has developed a robust, […]
BellBrook Labs
1232 Fourier Drive, Suite 115
Madison, Wisconsin 53717 USA
(608) 443-2400
info@bellbrooklabs.com