Summary Fluorescence polarization (FP) is a powerful biophysical technique used to study molecular interactions in solution — from protein–ligand binding to enzyme kinetics. Its homogenous, mix-and-read format makes FP assays ideal for high-throughput screening (HTS) in drug discovery. BellBrook Labs’ Transcreener® FP Assays leverage this principle to accelerate hit identification and lead optimization across diverse […]
Quick Answer: What Is an Enzyme Assay and Why It Matters An enzyme assay is a laboratory method used to measure the activity of an enzyme—how efficiently it converts a substrate into a product. Enzyme assays are fundamental tools in drug discovery, enabling researchers to identify, quantify, and characterize how small molecules modulate enzyme activity. […]
High-throughput screening (HTS) is a cornerstone of modern drug discovery. By enabling rapid, large-scale testing of chemical libraries against biological targets, HTS provides a powerful path to identify active compounds, validate drug targets, and accelerate hit-to-lead development. At BellBrook Labs, we specialize in biochemical high-throughput screening assays that are simple, robust, and designed for high-content […]
Introduction Adenosine triphosphate (ATP) is the universal energy currency of the cell, driving biochemical reactions across nearly all forms of life. Many enzymes rely on the hydrolysis of ATP to adenosine diphosphate (ADP) to fuel processes such as molecular transport, macromolecule synthesis, and mechanical motion. The enzymes that catalyze this hydrolysis are collectively known as […]
Summary Biochemical assay development is the process of designing, optimizing, and validating methods to measure enzyme activity, binding, or functional outcomes. It’s a cornerstone of preclinical research—enabling scientists to screen compounds, study mechanisms, and evaluate drug candidates. By combining proven universal assay platforms such as Transcreener® and AptaFluor®, with sound assay optimization strategies, researchers can […]
Summary Preclinical development bridges the gap between discovery and clinical testing by validating drug candidates through precise biochemical and cell-based assays. BellBrook Labs’ Transcreener® and AptaFluor® assay platforms empower researchers to streamline preclinical drug development by delivering robust, HTS-ready biochemical assays that quantify key enzyme activities, accelerate mechanism studies, and reduce false positives. These assays […]
Summary Artificial intelligence (AI) and automation are revolutionizing drug discovery by accelerating hit identification, improving lead optimization, and reducing costs across R&D workflows. Through predictive modeling, automated screening, and robotic experimentation, researchers can now design and validate drug candidates in a fraction of the time. Together, AI and automation are creating a new era of […]
Summary Biochemical assays are essential tools for quantifying enzyme activity, binding interactions, and compound potency in drug discovery. However, developing reliable assays can be challenging due to issues such as signal variability, reagent instability, interference from compounds, and poor assay robustness. By optimizing assay design, detection methods, and controls, researchers can overcome these challenges and […]
Summary ATPases are enzymes that hydrolyze adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and inorganic phosphate, releasing energy that drives essential biological processes. From muscle contraction to ion transport and protein folding, ATPases convert chemical energy into mechanical or biochemical work. In drug discovery, ATPase assays—such as the Transcreener® ADP² ATPase Assay—enable researchers to measure […]
Introduction In modern drug discovery, biochemical assay platforms are essential for identifying and characterizing enzyme modulators. Among the most biologically and therapeutically significant enzymes is SIRT1 (sirtuin 1), a NAD⁺-dependent deacetylase that regulates key cellular processes including metabolism, aging, inflammation, and epigenetic control. For scientists working in high-throughput screening (HTS) and epigenetic drug discovery, a […]
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